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scr control aav plasmid constructs  (Addgene inc)


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    Structured Review

    Addgene inc scr control aav plasmid constructs
    Scr Control Aav Plasmid Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1091 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+aav+construct/scramble+shRNA+(Plasmid+%231864)/pmc12541345-199-7-16
    Average 96 stars, based on 1091 article reviews
    scr control aav plasmid constructs - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Bioprocessing:

    Article Title: Histone Lysine Crotonylation Regulates Long-Term Memory Storage
    Article Snippet: AAV 9 -CaMKIIα-EGFP and AAV 9 -CaMKIIα-CDYL-V5 were purchased from VectorBuilder (VectorBuilder Inc). .. AAV 9 -CaMKIIα-GFP-Cre was purchased from Addgene (#105551). ..

    Article Title: Rapid neonatal AAV delivery for adult cortical two-photon imaging of genetically encoded sensors
    Article Snippet: .. In , AAV9- CAG -FLEX-GCaMP6f (Addgene, 100835; 100 nL of AAV per mouse) targeted to the neonatal prefrontal cortex (∼0.5–1 mm anterior to bregma, 0.5 mm lateral to midline) induced GCaMP6f expression in Rbp4-Cre (GENSAT project at Rockefeller University, 031125), PV-IRES-Cre (Jackson Laboratory, 008069), Sst-IRES-Cre (Jackson Laboratory, 013044), and VIP-IRES-Cre (Jackson Laboratory, 010908) mice. ..

    Article Title: Astrocytic TCF7L2 Impacts Brain Osmoregulation and Restricts Neuronal Excitability
    Article Snippet: Finally, slices were mounted on glass slides with Fluoromount G. Images were captured with an Axio Imager Z2 LSM 700 Zeiss confocal microscope. .. pZac2.1gfaABC1D‐tdTomato plasmid (TdTomato plasmid) was purchased from Addgene. pAAV:ITR‐U6‐sgRNA(anti‐ lacZ )‐gfaABC1D‐Cre (control) and pAAV:ITR‐U6‐sgRNA‐(anti‐ Tcf7l2 )‐gfaABC1D‐Cre ( Tcf7l2 KO) transfer plasmids were constructed on a backbone of AAV:ITR‐U6‐sgRNA(backbone)‐hSyn‐Cre‐2A‐EGFP‐KASH‐WPRE‐shortPA‐ITR (Addgene, catalog no. 60231) (Platt et al. ), from which 2A‐EGFP‐KASH sequences were removed and the hSyn promoter was replaced by gfaABC1D that was cloned from pZac2.1 gfaABC1D‐tdTomato (Addgene, catalog no. 44332) (Shigetomi et al. ). ..

    Article Title: In vivo Treatment of a Severe Vascular Disease via a Bespoke CRISPR-Cas9 Base Editor
    Article Snippet: Expression plasmids for human U6 promoter-driven gRNAs were generated by annealing and ligating duplexed oligonucleotides corresponding to spacer sequences into BsmBI-digested pUC19-U6-BsmBI_cassette-SpCas9_gRNA (BPK1520; Addgene plasmid 65777). .. Npu intein-split ABE constructs were cloned into N-and C-terminal AAV plasmids (Addgene plasmids 137177 and 137178, respectively). ..

    Construct:

    Article Title: Motor learning and dopamine-dependent striatal synaptic plasticity are controlled by astrocytic MEGF10
    Article Snippet: To generate pAAV- mDlx-DIO-PSD95.FingR-mCherry-eGFP-CCR5TC construct, PSD95.FingR-mCherry-eGFP construct from pAAV- mDlx-PSD95.FingR-mCherry-eGFP-CCR5TC and DIO construct from pAAV- EF1a-DIO-EYFP (Addgene #27056) were amplified by PCR, and both were subcloned into pAAV- mDlx-GFP-Fishell-1 . .. To generate the pAAV- GfaABC1D-GFP construct, the eGFP construct from pAAV- CAG-GFP (Addgene #28014) were amplified by PCR and subcloned into pAAV- GfaABC1D-Cre (Addgene #1056063). .. To generate pAAV- rTH-HA-hM3Dq and pAAV- rTH-HA-hM4Di constructs, HA-hM3Dq and HA-hM4Di construct from pAAV- GFAP-HA-hM3D(Gq)-IRES-mCitrine (Addgene #50470) and pAAV- GFAP-HA-hM4D(Gi)-IRES-mCitrine (Addgene #50471), respectively, were amplified by PCR and subcloned into pAAV- rTH-PI-Cre-SV40 (Addgene #107788).

    Article Title: Astrocytic TCF7L2 Impacts Brain Osmoregulation and Restricts Neuronal Excitability
    Article Snippet: Finally, slices were mounted on glass slides with Fluoromount G. Images were captured with an Axio Imager Z2 LSM 700 Zeiss confocal microscope. .. pZac2.1gfaABC1D‐tdTomato plasmid (TdTomato plasmid) was purchased from Addgene. pAAV:ITR‐U6‐sgRNA(anti‐ lacZ )‐gfaABC1D‐Cre (control) and pAAV:ITR‐U6‐sgRNA‐(anti‐ Tcf7l2 )‐gfaABC1D‐Cre ( Tcf7l2 KO) transfer plasmids were constructed on a backbone of AAV:ITR‐U6‐sgRNA(backbone)‐hSyn‐Cre‐2A‐EGFP‐KASH‐WPRE‐shortPA‐ITR (Addgene, catalog no. 60231) (Platt et al. ), from which 2A‐EGFP‐KASH sequences were removed and the hSyn promoter was replaced by gfaABC1D that was cloned from pZac2.1 gfaABC1D‐tdTomato (Addgene, catalog no. 44332) (Shigetomi et al. ). ..

    Article Title: In vivo Treatment of a Severe Vascular Disease via a Bespoke CRISPR-Cas9 Base Editor
    Article Snippet: Expression plasmids for human U6 promoter-driven gRNAs were generated by annealing and ligating duplexed oligonucleotides corresponding to spacer sequences into BsmBI-digested pUC19-U6-BsmBI_cassette-SpCas9_gRNA (BPK1520; Addgene plasmid 65777). .. Npu intein-split ABE constructs were cloned into N-and C-terminal AAV plasmids (Addgene plasmids 137177 and 137178, respectively). ..

    Amplification:

    Article Title: Motor learning and dopamine-dependent striatal synaptic plasticity are controlled by astrocytic MEGF10
    Article Snippet: To generate pAAV- mDlx-DIO-PSD95.FingR-mCherry-eGFP-CCR5TC construct, PSD95.FingR-mCherry-eGFP construct from pAAV- mDlx-PSD95.FingR-mCherry-eGFP-CCR5TC and DIO construct from pAAV- EF1a-DIO-EYFP (Addgene #27056) were amplified by PCR, and both were subcloned into pAAV- mDlx-GFP-Fishell-1 . .. To generate the pAAV- GfaABC1D-GFP construct, the eGFP construct from pAAV- CAG-GFP (Addgene #28014) were amplified by PCR and subcloned into pAAV- GfaABC1D-Cre (Addgene #1056063). .. To generate pAAV- rTH-HA-hM3Dq and pAAV- rTH-HA-hM4Di constructs, HA-hM3Dq and HA-hM4Di construct from pAAV- GFAP-HA-hM3D(Gq)-IRES-mCitrine (Addgene #50470) and pAAV- GFAP-HA-hM4D(Gi)-IRES-mCitrine (Addgene #50471), respectively, were amplified by PCR and subcloned into pAAV- rTH-PI-Cre-SV40 (Addgene #107788).

    Polymerase Chain Reaction:

    Article Title: Motor learning and dopamine-dependent striatal synaptic plasticity are controlled by astrocytic MEGF10
    Article Snippet: To generate pAAV- mDlx-DIO-PSD95.FingR-mCherry-eGFP-CCR5TC construct, PSD95.FingR-mCherry-eGFP construct from pAAV- mDlx-PSD95.FingR-mCherry-eGFP-CCR5TC and DIO construct from pAAV- EF1a-DIO-EYFP (Addgene #27056) were amplified by PCR, and both were subcloned into pAAV- mDlx-GFP-Fishell-1 . .. To generate the pAAV- GfaABC1D-GFP construct, the eGFP construct from pAAV- CAG-GFP (Addgene #28014) were amplified by PCR and subcloned into pAAV- GfaABC1D-Cre (Addgene #1056063). .. To generate pAAV- rTH-HA-hM3Dq and pAAV- rTH-HA-hM4Di constructs, HA-hM3Dq and HA-hM4Di construct from pAAV- GFAP-HA-hM3D(Gq)-IRES-mCitrine (Addgene #50470) and pAAV- GFAP-HA-hM4D(Gi)-IRES-mCitrine (Addgene #50471), respectively, were amplified by PCR and subcloned into pAAV- rTH-PI-Cre-SV40 (Addgene #107788).

    Expressing:

    Article Title: Rapid neonatal AAV delivery for adult cortical two-photon imaging of genetically encoded sensors
    Article Snippet: .. In , DREADD-induced modulation of pyramidal neurons was accomplished with expression of Cre-dependent DREADD-hM 3 D(G q ) (AAV9- hSYN -DIO-hM 3 D(G q )-mCherry; Addgene, 44361) under the human synapsin-1 promoter in Cre-positive mice or mice coexpressing AAV9- CaMK2 -Cre (Addgene, 105558). .. Pyramidal neuron activity was imaged before and ∼15-20 minutes after CNO i.p. injection (Tocris, 4936; 3 mg/kg; solution of CNO in saline 0.3 mg/ml).

    Article Title: Rapid neonatal AAV delivery for adult cortical two-photon imaging of genetically encoded sensors
    Article Snippet: .. In , AAV9- CAG -FLEX-GCaMP6f (Addgene, 100835; 100 nL of AAV per mouse) targeted to the neonatal prefrontal cortex (∼0.5–1 mm anterior to bregma, 0.5 mm lateral to midline) induced GCaMP6f expression in Rbp4-Cre (GENSAT project at Rockefeller University, 031125), PV-IRES-Cre (Jackson Laboratory, 008069), Sst-IRES-Cre (Jackson Laboratory, 013044), and VIP-IRES-Cre (Jackson Laboratory, 010908) mice. ..

    Plasmid Preparation:

    Article Title: Astrocytic TCF7L2 Impacts Brain Osmoregulation and Restricts Neuronal Excitability
    Article Snippet: Finally, slices were mounted on glass slides with Fluoromount G. Images were captured with an Axio Imager Z2 LSM 700 Zeiss confocal microscope. .. pZac2.1gfaABC1D‐tdTomato plasmid (TdTomato plasmid) was purchased from Addgene. pAAV:ITR‐U6‐sgRNA(anti‐ lacZ )‐gfaABC1D‐Cre (control) and pAAV:ITR‐U6‐sgRNA‐(anti‐ Tcf7l2 )‐gfaABC1D‐Cre ( Tcf7l2 KO) transfer plasmids were constructed on a backbone of AAV:ITR‐U6‐sgRNA(backbone)‐hSyn‐Cre‐2A‐EGFP‐KASH‐WPRE‐shortPA‐ITR (Addgene, catalog no. 60231) (Platt et al. ), from which 2A‐EGFP‐KASH sequences were removed and the hSyn promoter was replaced by gfaABC1D that was cloned from pZac2.1 gfaABC1D‐tdTomato (Addgene, catalog no. 44332) (Shigetomi et al. ). ..

    Control:

    Article Title: Astrocytic TCF7L2 Impacts Brain Osmoregulation and Restricts Neuronal Excitability
    Article Snippet: Finally, slices were mounted on glass slides with Fluoromount G. Images were captured with an Axio Imager Z2 LSM 700 Zeiss confocal microscope. .. pZac2.1gfaABC1D‐tdTomato plasmid (TdTomato plasmid) was purchased from Addgene. pAAV:ITR‐U6‐sgRNA(anti‐ lacZ )‐gfaABC1D‐Cre (control) and pAAV:ITR‐U6‐sgRNA‐(anti‐ Tcf7l2 )‐gfaABC1D‐Cre ( Tcf7l2 KO) transfer plasmids were constructed on a backbone of AAV:ITR‐U6‐sgRNA(backbone)‐hSyn‐Cre‐2A‐EGFP‐KASH‐WPRE‐shortPA‐ITR (Addgene, catalog no. 60231) (Platt et al. ), from which 2A‐EGFP‐KASH sequences were removed and the hSyn promoter was replaced by gfaABC1D that was cloned from pZac2.1 gfaABC1D‐tdTomato (Addgene, catalog no. 44332) (Shigetomi et al. ). ..

    Clone Assay:

    Article Title: Astrocytic TCF7L2 Impacts Brain Osmoregulation and Restricts Neuronal Excitability
    Article Snippet: Finally, slices were mounted on glass slides with Fluoromount G. Images were captured with an Axio Imager Z2 LSM 700 Zeiss confocal microscope. .. pZac2.1gfaABC1D‐tdTomato plasmid (TdTomato plasmid) was purchased from Addgene. pAAV:ITR‐U6‐sgRNA(anti‐ lacZ )‐gfaABC1D‐Cre (control) and pAAV:ITR‐U6‐sgRNA‐(anti‐ Tcf7l2 )‐gfaABC1D‐Cre ( Tcf7l2 KO) transfer plasmids were constructed on a backbone of AAV:ITR‐U6‐sgRNA(backbone)‐hSyn‐Cre‐2A‐EGFP‐KASH‐WPRE‐shortPA‐ITR (Addgene, catalog no. 60231) (Platt et al. ), from which 2A‐EGFP‐KASH sequences were removed and the hSyn promoter was replaced by gfaABC1D that was cloned from pZac2.1 gfaABC1D‐tdTomato (Addgene, catalog no. 44332) (Shigetomi et al. ). ..

    Article Title: In vivo Treatment of a Severe Vascular Disease via a Bespoke CRISPR-Cas9 Base Editor
    Article Snippet: Expression plasmids for human U6 promoter-driven gRNAs were generated by annealing and ligating duplexed oligonucleotides corresponding to spacer sequences into BsmBI-digested pUC19-U6-BsmBI_cassette-SpCas9_gRNA (BPK1520; Addgene plasmid 65777). .. Npu intein-split ABE constructs were cloned into N-and C-terminal AAV plasmids (Addgene plasmids 137177 and 137178, respectively). ..

    Labeling:

    Article Title: Rapid neonatal AAV delivery for adult cortical two-photon imaging of genetically encoded sensors
    Article Snippet: .. In , , and , pyramidal neurons were labeled using recombinant AAV9- CaMK2 -Cre (Addgene, 105558), AAV9- CAG -FLEX-tdTomato (Addgene, 28306), AAV9- CAG -FLEX-GCaMP6f (Addgene, 100835), and/or AAV9- hSYN -DIO-mFos-eGFP-WPRE (Vector Biolabs, 1.68 x 10 13 GC/mL, Lot# 230522#59). .. Different labeling densities were accomplished by diluting Cre virus in artificial cerebrospinal fluid (aCSF)/fast-green dye (Sigma Aldrich, F725) solution followed by mixing with GCaMP6f and tdTomato viruses.

    Recombinant:

    Article Title: Rapid neonatal AAV delivery for adult cortical two-photon imaging of genetically encoded sensors
    Article Snippet: .. In , , and , pyramidal neurons were labeled using recombinant AAV9- CaMK2 -Cre (Addgene, 105558), AAV9- CAG -FLEX-tdTomato (Addgene, 28306), AAV9- CAG -FLEX-GCaMP6f (Addgene, 100835), and/or AAV9- hSYN -DIO-mFos-eGFP-WPRE (Vector Biolabs, 1.68 x 10 13 GC/mL, Lot# 230522#59). .. Different labeling densities were accomplished by diluting Cre virus in artificial cerebrospinal fluid (aCSF)/fast-green dye (Sigma Aldrich, F725) solution followed by mixing with GCaMP6f and tdTomato viruses.



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